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pas2 1 yeast expression vector  (TaKaRa)


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    Structured Review

    TaKaRa pas2 1 yeast expression vector
    Pas2 1 Yeast Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yeast+expression+vector+pas2+1/pm12059041-35-11-15
    Average 86 stars, based on 1 article reviews
    pas2 1 yeast expression vector - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Isolation:

    Article Title: Identification of a Novel Human Tankyrase through Its Interaction with the Adaptor Protein Grb14
    Article Snippet: .. The 1.7-kb fragment was isolated and cloned into the NdeI (Klenowtreated) and BamHI sites of the yeast expression vector pAS2–1 (CLONTECH, Palo Alto, CA) to generate GRB14/pAS2–1 containing an in-frame fusion of full-length Grb14 with the Gal4 DNA-BD. ..

    Clone Assay:

    Article Title: Identification of a Novel Human Tankyrase through Its Interaction with the Adaptor Protein Grb14
    Article Snippet: .. The 1.7-kb fragment was isolated and cloned into the NdeI (Klenowtreated) and BamHI sites of the yeast expression vector pAS2–1 (CLONTECH, Palo Alto, CA) to generate GRB14/pAS2–1 containing an in-frame fusion of full-length Grb14 with the Gal4 DNA-BD. ..

    Article Title: Tumor Necrosis Factor Receptor 1 Is an ATPase Regulated by Silencer of Death Domain
    Article Snippet: .. A cDNA encoding the full-length testis-specific HSC70t mouse protein ( 24 , 25 ) was cloned into the yeast expression vector pAS2-1 (Clontech) and used for screening a mouse testis cDNA library (Clontech) as described previously ( 26 ). ..

    Expressing:

    Article Title: Identification of a Novel Human Tankyrase through Its Interaction with the Adaptor Protein Grb14
    Article Snippet: .. The 1.7-kb fragment was isolated and cloned into the NdeI (Klenowtreated) and BamHI sites of the yeast expression vector pAS2–1 (CLONTECH, Palo Alto, CA) to generate GRB14/pAS2–1 containing an in-frame fusion of full-length Grb14 with the Gal4 DNA-BD. ..

    Article Title: Insertional Mutation of the Murine Kisimo Locus Caused a Defect in Spermatogenesis
    Article Snippet: .. A cDNA encoding fulllength mouse THEG was subcloned into the yeast expression vector pAS2–1 (CLONTECH) fused in frame with the DNA binding domain of yeast transcriptional activator GAL4, which generated pAS2–1mTHEG1b. ..

    Article Title: Specific domain of cGMP-dependent protein kinase Ibeta but not Ialpha functions as a transcriptional activator in yeast.
    Article Snippet: Recently, cyclic GMP-dependent protein kinase (cGK) was shown to translocate to the nucleus and regulate gene transcription.. To determine whether cGK I proteins function as transcriptional activators, we produced the constructs of cGK I® or I ̄ fused with the DNA binding domain of the yeast transcriptional activator GAL4.. Here, we demonstrate that the amino-terminal region of cGK I ̄ (amino acids 1–107) exhibits transcriptional activation in yeast.

    Article Title: Binding and phosphorylation of a novel male germ cell-specific cGMP-dependent protein kinase-anchoring protein by cGMP-dependent protein kinase Ialpha.
    Article Snippet: .. A cDNA encoding full-length cGK-Ia or its N-terminal region (amino acids 1–416) was subcloned into the BamHI (made blunt with T4 DNA polymerase) site of the yeast expression vector pAS2-1 (CLONTECH) fused in frame with the DNA-binding domain of the yeast transcriptional activator GAL4, generating pAS2-1-cGK-Ia or pAS2-1cGK-IaN, respectively. ..

    Article Title: The nonhelical tail domain of keratin 14 promotes filament bundling and enhances the mechanical properties of keratin intermediate filaments in vitro
    Article Snippet: .. PCR products corresponding to the human K14 tail domain, starting at 425 His , and the K16 tail domain, starting at 426 Ser , were fused in frame to the GAL4 DNA binding domain in the yeast expression vector pAS2–1 (ClONTECH Laboratories, Inc.). ..

    Article Title: Tumor Necrosis Factor Receptor 1 Is an ATPase Regulated by Silencer of Death Domain
    Article Snippet: .. A cDNA encoding the full-length testis-specific HSC70t mouse protein ( 24 , 25 ) was cloned into the yeast expression vector pAS2-1 (Clontech) and used for screening a mouse testis cDNA library (Clontech) as described previously ( 26 ). ..

    Article Title: Regulation of matrix attachment region-dependent, lymphocyte-restricted transcription through differential localization within promyelocytic leukemia nuclear bodies
    Article Snippet: .. The Bright coding sequence (216–701) was inserted in yeast expression vector pAS2-1 (Clontech). ..

    Plasmid Preparation:

    Article Title: Insertional Mutation of the Murine Kisimo Locus Caused a Defect in Spermatogenesis
    Article Snippet: .. A cDNA encoding fulllength mouse THEG was subcloned into the yeast expression vector pAS2–1 (CLONTECH) fused in frame with the DNA binding domain of yeast transcriptional activator GAL4, which generated pAS2–1mTHEG1b. ..

    Article Title: Specific domain of cGMP-dependent protein kinase Ibeta but not Ialpha functions as a transcriptional activator in yeast.
    Article Snippet: Recently, cyclic GMP-dependent protein kinase (cGK) was shown to translocate to the nucleus and regulate gene transcription.. To determine whether cGK I proteins function as transcriptional activators, we produced the constructs of cGK I® or I ̄ fused with the DNA binding domain of the yeast transcriptional activator GAL4.. Here, we demonstrate that the amino-terminal region of cGK I ̄ (amino acids 1–107) exhibits transcriptional activation in yeast.

    Article Title: Binding and phosphorylation of a novel male germ cell-specific cGMP-dependent protein kinase-anchoring protein by cGMP-dependent protein kinase Ialpha.
    Article Snippet: .. A cDNA encoding full-length cGK-Ia or its N-terminal region (amino acids 1–416) was subcloned into the BamHI (made blunt with T4 DNA polymerase) site of the yeast expression vector pAS2-1 (CLONTECH) fused in frame with the DNA-binding domain of the yeast transcriptional activator GAL4, generating pAS2-1-cGK-Ia or pAS2-1cGK-IaN, respectively. ..

    Article Title: The nonhelical tail domain of keratin 14 promotes filament bundling and enhances the mechanical properties of keratin intermediate filaments in vitro
    Article Snippet: .. PCR products corresponding to the human K14 tail domain, starting at 425 His , and the K16 tail domain, starting at 426 Ser , were fused in frame to the GAL4 DNA binding domain in the yeast expression vector pAS2–1 (ClONTECH Laboratories, Inc.). ..

    Article Title: Tumor Necrosis Factor Receptor 1 Is an ATPase Regulated by Silencer of Death Domain
    Article Snippet: .. A cDNA encoding the full-length testis-specific HSC70t mouse protein ( 24 , 25 ) was cloned into the yeast expression vector pAS2-1 (Clontech) and used for screening a mouse testis cDNA library (Clontech) as described previously ( 26 ). ..

    Article Title: Regulation of matrix attachment region-dependent, lymphocyte-restricted transcription through differential localization within promyelocytic leukemia nuclear bodies
    Article Snippet: .. The Bright coding sequence (216–701) was inserted in yeast expression vector pAS2-1 (Clontech). ..

    Binding Assay:

    Article Title: Insertional Mutation of the Murine Kisimo Locus Caused a Defect in Spermatogenesis
    Article Snippet: .. A cDNA encoding fulllength mouse THEG was subcloned into the yeast expression vector pAS2–1 (CLONTECH) fused in frame with the DNA binding domain of yeast transcriptional activator GAL4, which generated pAS2–1mTHEG1b. ..

    Article Title: The nonhelical tail domain of keratin 14 promotes filament bundling and enhances the mechanical properties of keratin intermediate filaments in vitro
    Article Snippet: .. PCR products corresponding to the human K14 tail domain, starting at 425 His , and the K16 tail domain, starting at 426 Ser , were fused in frame to the GAL4 DNA binding domain in the yeast expression vector pAS2–1 (ClONTECH Laboratories, Inc.). ..

    Generated:

    Article Title: Insertional Mutation of the Murine Kisimo Locus Caused a Defect in Spermatogenesis
    Article Snippet: .. A cDNA encoding fulllength mouse THEG was subcloned into the yeast expression vector pAS2–1 (CLONTECH) fused in frame with the DNA binding domain of yeast transcriptional activator GAL4, which generated pAS2–1mTHEG1b. ..

    Article Title: Specific domain of cGMP-dependent protein kinase Ibeta but not Ialpha functions as a transcriptional activator in yeast.
    Article Snippet: Recently, cyclic GMP-dependent protein kinase (cGK) was shown to translocate to the nucleus and regulate gene transcription.. To determine whether cGK I proteins function as transcriptional activators, we produced the constructs of cGK I® or I ̄ fused with the DNA binding domain of the yeast transcriptional activator GAL4.. Here, we demonstrate that the amino-terminal region of cGK I ̄ (amino acids 1–107) exhibits transcriptional activation in yeast.

    Construct:

    Article Title: Specific domain of cGMP-dependent protein kinase Ibeta but not Ialpha functions as a transcriptional activator in yeast.
    Article Snippet: Recently, cyclic GMP-dependent protein kinase (cGK) was shown to translocate to the nucleus and regulate gene transcription.. To determine whether cGK I proteins function as transcriptional activators, we produced the constructs of cGK I® or I ̄ fused with the DNA binding domain of the yeast transcriptional activator GAL4.. Here, we demonstrate that the amino-terminal region of cGK I ̄ (amino acids 1–107) exhibits transcriptional activation in yeast.

    Polymerase Chain Reaction:

    Article Title: The nonhelical tail domain of keratin 14 promotes filament bundling and enhances the mechanical properties of keratin intermediate filaments in vitro
    Article Snippet: .. PCR products corresponding to the human K14 tail domain, starting at 425 His , and the K16 tail domain, starting at 426 Ser , were fused in frame to the GAL4 DNA binding domain in the yeast expression vector pAS2–1 (ClONTECH Laboratories, Inc.). ..

    cDNA Library Assay:

    Article Title: Tumor Necrosis Factor Receptor 1 Is an ATPase Regulated by Silencer of Death Domain
    Article Snippet: .. A cDNA encoding the full-length testis-specific HSC70t mouse protein ( 24 , 25 ) was cloned into the yeast expression vector pAS2-1 (Clontech) and used for screening a mouse testis cDNA library (Clontech) as described previously ( 26 ). ..

    Sequencing:

    Article Title: Regulation of matrix attachment region-dependent, lymphocyte-restricted transcription through differential localization within promyelocytic leukemia nuclear bodies
    Article Snippet: .. The Bright coding sequence (216–701) was inserted in yeast expression vector pAS2-1 (Clontech). ..



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